Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Filter Lens, 630 nm, Emission
The 630 nm filter lens is an interchangeable emission filter designed to enable red-region fluorescence detection on compatible microplate readers. It provides selective transmission centered at 630 nm to improve signal specificity for assays using red-emitting fluorophores.
- Center wavelength 630 nm.
- Designed for emission detection in the red spectral region.
- Interchangeable filter format for compatible microplate readers.
- Enhances signal specificity and reduces background for fluorescence assays.
- Single-unit form factor suitable for routine assay configuration changes.
Molecular Devices SpectraTest Validation Plate, FL1, Calibration Certified, Reader Compatible
The SpectraTest FL1 Fluorescence Validation Plate is a calibrated reference tool used to validate the optical performance of FL1-channel fluorescence-capable microplate readers. It verifies sensitivity, wavelength accuracy, RFU linearity, PMT matching, and other fluorescence performance parameters and ships with a certificate of calibration and protective storage.
- Validates fluorescence sensitivity, wavelength accuracy, PMT matching, and RFU linearity.
- Includes calibrated fluorescent and non-fluorescent reference strips and didymium glass.
- Supplied with a certificate of calibration and protective storage sleeve.
- Compatible with a wide range of microplate readers for FL1-channel testing.
- Recommended annual recertification to maintain traceable calibration.
Molecular Devices Low-Volume Slide Refill Kit, 64-Well, Pack of 3
Replenishment kit of low-volume bottom slides for slide-based microplate readers. The kit includes three low-volume bottom slides, each with 64 wells, intended for low-volume absorbance and related assays.
- Contains three 64-well low-volume bottom slides.
- Intended for low-volume absorbance and slide-based assays.
- Supplies replacement slides to restore slide reader capacity.
Electron Microscopy Sciences EMS SmartDoc™ 590nm Band Pass Filter for EtBr
For use with EMS SmartDoc gel imaging system.
Revvity Health Sciences Inc HTRF Human IL-12/IL-23 p40 Detection Kit, 100,000 Assay Points
HTRF Human IL-12/IL-23 p40 Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATF4 Detection Kit, 50,000 Assay Points
HTRF Human Total ATF4 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse GAPDH Housekeeping Kit, 50,000 Assay Points
HTRF Human & Mouse GAPDH Housekeeping Kit, 50,000 Assay Points
Medchemexpress LLC Tofacitinib 10 mM * 1 mL in DMSO, Solid + Solvent, Ready for Reconstitution
Tofacitinib is an orally available JAK3/2/1 inhibitor with IC50s of 1, 20, and 112 nM, respectively.
Revvity Health Sciences Inc HTRF Human Total PINK1 Detection Kit, 500 Assay Points
HTRF Human Total PINK1 Detection Kit, 500 Assay Points
Molecular Devices Filter Slide, Empty, Excitation, 6-Filter Capacity
Empty excitation filter slide for FilterMax multi-mode microplate readers. Supplied unpopulated to allow custom installation of excitation filters for fluorescence and many luminescence methods. The slide installs into the instrument filter track and secures filters with small hex screws.
- Supplied empty for custom filter configurations.
- Accepts up to six individual filters per slide.
- Distinct sizes for excitation and emission slides to prevent misinstallation.
- Install and handle by the tab to avoid contaminating filter surfaces.
- When configured as an excitation slide, not compatible with fluorescence polarization (FP) methods.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Revvity Health Sciences Inc HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points
HTRF Human IL-23 / IL12R-β1 Binding Kit, 10,000 Assay Points